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KMID : 0545119970070050352
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Journal of Microbiology and Biotechnology
1997 Volume.7 No. 5 p.352 ~ p.355
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Measurement of Iron-dependence of pupA Promoter Activity by a pup-lux Bioreporter
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KHANG, YONG-HO
YANG, ZAMIN K./BURLAGE, ROBERT S.
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Abstract
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The promoter region of the pupA gene of Pseudomonas putida WCS358 was fused with the structural genes for bioluminescence (luxCDABE) from Vibrio fischeri, and the resulting fusion plasmid harbored by the WCS358 host. The pup-lux fusion gene was then used for quantitative analysis of the iron-dependence of pupA promoter activity. Factors affecting bioluminescence produced by the pup-lux bioreporter were found to be cell activity, iron-chelator concentrations, Fe(Ⅲ) concentrations, and nutrient components. Light production rates of the pup-lux bioreporter were inversely dependent upon iron molecules when FeCl_3 concentrations were between 10^-2 and 1 μM in nutrient-poor minimal media, and between 0.1 and 10 mM in nutrient-rich complex media.
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